Services

Sistemas Genómicos has an interdisciplinary group of bioinformatics experts specialised in analysing data from high-performance platforms.

The constant development of high-performance sequencing platforms has given rise to a swift increase in the amount of genomic data generated. Unlike traditional sequencing systems, these platforms are capable of generating, in parallel and on a massive scale, millions of DNA fragments in one sole sequencing process in record timing and at a cost that is getting lower and lower. Due to its amazing performance, this type of platform is perfect for conducting an infinite amount of large-scale studies that could not be approached with any other existing technology today given the enormous cost this would involve.

Sistemas Genómicos currently offers analysis for any kind of application (exome, whole genome, smallRNAs, methylation, ChIP-Seq, RNA-Seq) and the most extensive next-generation sequencers (454-Roche, Solexa-Illumina, SOLiD-LifeTechnologies). Furthermore, the company has the equipment and staff necessary to provide additional support for your research, both for extracting genetic material from sample and for the final checks on the data.

Resequencing

Targeted Resequencing. Targeted resequencing involves isolating, enriching and sequencing specific regions of a genome in a sample. This technique allows for the systematic detection of common variants and rare or uncommon variants. Currently, the combination of systems for solution enrichment and massive sequencing has become the method of choice for selectively classifying a large number of genes simultaneously thanks to its high level of precision, reproducibility and performance.

  • Exome resequencing is an innovative technology which allows us to capture, enhance and sequence codifying genomic regions. Whole exome resequencing in humans allows us to identify new genes associated with both rare and common diseases.
  • Customised (gene panels, specific genome regions). Targeted resequencing allows for us to study specific regions of the genome using customised probes.

Whole genome. One of the most extensive applications of Next-Generation Sequencing (NGS) is the sequencing of whole genomes. The massive sequencing platforms are able of producing hundreds of gigabases in just one run, which has been helpful in developing huge sequencing projects, like the 1000 Genomes Project or the HapMap project, aimed at gathering knowledge about how genetic variations affect health and the development of diseases.

Transcriptomics

RNA – Seq – Whole Transcriptome. “RNA-Seq” is the name given to the massive sequencing of cDNA high-performance platforms aimed at obtaining universal information about the RNA content of a sample, including mRNAs, rRNAs, tRNAs and other non-codifying RNAs, made up of one cell or a set of cells. Thanks to the effectiveness and performance of the Next-Generation Sequencing platforms, the RNA-Seq technique provides researchers with an effective way of measuring the levels of gene expression, identifying alternative splicing events, identifying gene fusion events and identifying SNVs simultaneously while reducing the price per sample.

Serial Analysis of Gene Expression is a technique that allows for analysis of thousands of transcriptions at the same time using sequencing from the 5’-end or 3’-end of the transcription. This method allows us to identify and quantify the transcriptions. Unlike whole transcriptome sequencing, SAGE does not allow us to identify splicing events or fusion genes; however, it is a quicker and cheaper technique.

smallRNAs, are small non-codifying RNAs that contain a large number of molecules with various functions and structures (miRNAs, snoRNAs, piRNAs, etc).

ChIP-Seq

ChIP-Seq technology brings together chromatin immunoprecipitation and massive sequencing, allowing for the identification of interaction zones between protein and DNA (cistrome).

Methylation

DNA methylation is a molecular process that is essential for the development of higher organisms. The study of methylation patterns is widely used in research on the regulation of essential processes such as cell differentiation, development or the appearance of diseases. New massive sequencing technology allows us to identify universal methylation patterns (methylome) with a high level of reliability very quickly and at a very low cost per base compared to traditional Sanger sequencing.

Microarray data analysis: Expression microarrays allow us to examine a large number of genes at the same time in order to determine their level of expression in a specific cell type for a specific time and/or condition. This technology is widely used both in basic research and in clinical trials.

Our Bioinformatics group has broad experience in the analysis of data from microarrays of the main commercial brands (Affymetrix, Agilent and Illumina).

Sistemas Genómicos will provide you with the support necessary to successfully complete your research project, not only providing you with a good analysis service but also assisting you with the experimental validation of the results.

Most genomics or transcriptomics projects currently involve the processing of complex data, which requires qualified staff.

The Bioinformatics team is able to carry out tailor-made projects to analyse data from high-performance and cutting-edge technology.

Our experience in various areas of research allows us to advise our customers from the initial concept of the project up to drawing conclusions from it.

Similarly, our business has advanced technological equipment and highly specialised staff in various research areas with whom you can develop additional studies that back up your results in order to successfully complete your project.